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Image Search Results
Journal: Biomimetics
Article Title: The Biomimetics of Mg 2+ -Concentration-Resolved Microenvironment for Bone and Cartilage Repairing Materials Design
doi: 10.3390/biomimetics7040227
Figure Lengend Snippet: Osteogenic-related gene expressions of MC3T3-E1 cells cultured in different concentrations of Mg 2+ ( A1 – A4 ), all data are presented as fold change after normalization to GAPDH, and fold changes are shown as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001. COL I contents of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by ELISA ( B ). ALP activity of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by quantitative ALP activity assay ( C1 ) and ALP activity staining assay ( C2 , C3 ). Effect of Mg 2+ concentrations on the formation of calcium nodules ( D1 , D2 ). The scale bars in C2, C3, D1, and D2 are 5 mm, 200 μm, 5 mm, and 200 μm, respectively.
Article Snippet: Rabbit Collagen Type II ELISA Kit (Cusabio, Wuhan, China),
Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Activity Assay, ALP Activity Assay, Staining